Description
Description: This assay employs a two-site sandwich ELISA to quantitate LAPTM4A in samples. An antibody specific for LAPTM4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAPTM4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAPTM4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAPTM4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: LAPTM4a encodes a protein that has four predicted transmembrane domains. The function of this gene has not yet been determined; however, studies in the mouse homolog suggest a role in the transport of small molecules across endosomal and lysosomal membranes.Significant transmembrane domains were identified in 17 genes, and protein motifs that matched those in the PROSITE motif database were identified in 11 genes. Northern hybridization analysis with 18 different cells and tissues demonstrated that 10 genes were apparently expressed in a cell-specific or tissue-specific manner. Among the genes predicted, half were isolated from the medium-sized cDNA library and the other half from the small-sized cDNA library, and their average sizes were 4 kb and 1.4 kb, respectively